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GFP Sec16B is knocked down efficiently with siRNAs except Sec16B UTR which only targets the endogenous Sec16B.
Note have an effect on peripheral puncta labelled with Sec24C or ERGIC 53 when cells are depleted of Sec16A and not when depleted of Sec16B.
coli has a tendency to ensure lasting repair off the suppressor tRNA.
1010) recognized small puncta in NHDF cells.
as used in21, Sec16B UTR individuals 3′.
Additionally, while in front of Firefly luciferase we inserted trp attenuator region, while Renilla luciferase was created as internal control (Figure 2k, left panel).
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